ABSTRACT
OBJECTIVE@#To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell (hMSC) transplantation on the liver fibrosis in carbon tetrachloride-induced cirrhosis rats.@*METHODS@#Sixty SD rats were randomly divided into five groups. The rats in control group received intraperitoneal injection of saline, while those in model control group, treatment group A, group B and group C received intraperitoneal injection of carbon tetrachloride oily solution to induce liver cirrhosis within 8 weeks. Then, the rats in the model control group, treatment group A, treatment group B, treatment group C received vein tail injection of saline, Rougan Huaqian granules, hMSC suspension and Rougan Huaqian granules combined with hMSC suspension.@*RESULTS@#The treatment groups had significantly different liver function (AST levels), liver fibrosis index (laminin and HA), hepatic sinusoidal wallsα-smooth muscle actin, IV collagen and laminin protein expression and I, III collagen from the model group (P<0.05). The transplanted cells showed human hepatocyte-like cells differentiation trend in the liver.@*CONCLUSIONS@#The Rougan Huaqian granules combined with hMSC transplantation can alleviate liver fibrosis in cirrhosis rats.
Subject(s)
Animals , Female , Humans , Male , Rats , Body Weight , Collagen , Genetics , Metabolism , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Liver , Chemistry , Metabolism , Pathology , Liver Cirrhosis , Metabolism , Pathology , Therapeutics , Matrix Metalloproteinase 13 , Genetics , Metabolism , Mesenchymal Stem Cell Transplantation , Organ Size , Rats, Sprague-DawleyABSTRACT
OBJECTIVE@#To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats.@*METHODS@#A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected.@*RESULTS@#Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05).@*CONCLUSIONS@#BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.